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產品概述
| 名稱 | A549 [A-549] (人非小細胞肺癌細胞) (STR鑒定正確) |
| 別稱 | A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549 |
| 種屬 | 人類 |
| 年齡（性別） | 男性，58歲 |
| 組織來源 | 肺癌 |
| 生長特性 | 貼壁細胞 |
| 細胞形態 | 上皮細胞樣 |
| 背景描述 | A549細胞是由D·J·Griad通過肺癌組織移植培養建系，源自于一位58歲白人男性；A549能通過胞苷二磷酸膽堿途徑合成含有高含量不飽和脂肪酸的卵磷脂。 |
| 生物安全等級 | 1 |
| 生長培養基 | Ham's F-12K＋10% FBS＋1% P/S |
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2~3次/周 |
| 倍增時間 | ~22小時 |
| 凍存條件 | 凍存液：55% 基礎培養基+40%FBS+5%DMSO 溫度：液氮 |
| 培養條件 | 氣相：空氣，95%；CO2，5% 溫度：37℃ |
| 保藏機構 | ATCC; CCL-185 ATCC; CRM-CCL-185 ATCC; CRL-7909 BCRC; 60074 BCRJ; 0033 DSMZ; ACC-107 ECACC; 86012804 |
STR鑒定
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STR位點信息
Amelogenin X,Y CSF1PO 10,12 D2S1338 24 D3S1358 16 D5S818 11 D7S820 8,11 D8S1179 13,14 D13S317 11 D16S539 11,12 D18S51 14,17 D19S433 13 D21S11 29 FGA 23 PentaD 9 PentaE 7,11 TH01 8,9.3 TPOX 8,11 vWA 14 D6S1043 11,13 D12S391 18 D2S441 10,13 -
STR鑒定圖
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參考文獻
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Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase(2021/8/5)
作者：Shao, Ximing, Ding, Zhihao, Zhou, Wenhua
期刊：Nature Nanotechnology
DOI：10.1038/s41565-021-00952-x
影響因子 ：39.213
引用產品：CL-0016 CL-0101 CL-0103 CL-0150A CL-0150B CL-0236 CL-0238
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LINC00173.v1 Promotes Angiogenesis and Progression of Lung Squamous Cell Carcinoma by Sponging miR-511-5p to Regulate VEGFA Expression(2020.07.10)
作者：Aibin Liu
期刊：Molecular Cancer
DOI：10.1186/s12943-020-01217-2
影響因子 ：27.401
-
20(S)-Protopanaxatriol promotes the binding of P53 and DNA to regulate the antitumor network via multiomic analysis(June 2020)
作者：Zhihua Wang, Wenbo Wu, Xiangchen Guan
期刊：Acta Pharmaceutica Sinica B
DOI：10.1016/j.apsb.2020.01.017
影響因子 ：11.413?
引用產品：CL-0016
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MoS2 Quantum Dot@Polyaniline Inorganic–Organic Nanohybrids for in Vivo Dual-Modal Imaging Guided Synergistic Photothermal/Radiation Therapy(2016/9/7)
作者：Jinping Wang, Xiaoxiao Tan, Xiaojuan Pang
期刊：ACS Applied Materials & Interfaces
影響因子 ：9.228999999999999
引用產品：CL-0016 CL-0101 CL-0103 CL-0150A CL-0150B CL-0236 CL-0238
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The Immune Heterogeneity Between Pulmonary Adenocarcinoma and Squamous Cell Carcinoma: A Comprehensive Analysis Based on lncRNA Model(2021/07/29)
作者：Yan Tao, Ma Guoyuan, Wang Kai
期刊：Frontiers in Immunology
影響因子 ：7.561
引用產品：CL-0016
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miR-371b-5p-Engineered Exosomes Enhances Tumor Inhibitory Effect(2021/10/4)
作者：Xue Qiang, Yang Yang, Yang Linlin
期刊：Frontiers in Cell and Developmental Biology
影響因子 ：6.684
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Inhibition of miR-185-3p Confers Erlotinib Resistance Through Upregulation of PFKL/MET in Lung Cancers(2021/7/21)
作者：Li Ke, Zhu Xinling, Yuan Conghu
期刊：Frontiers in Cell and Developmental Biology
影響因子 ：6.684
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C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma(2021/7/7)
作者：Dong Xiaoxia, Liu Yongyu, Deng Xinzhou
期刊：Frontiers in Cell and Developmental Biology
影響因子 ：6.684
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Erianin inhibits human lung cancer cell growth via PI3K/Akt/mTOR pathway in vitro and in vivo(2021/3/17)
作者：Hui-qiong Zhang, Xiao-fang Xie, Gang-min Li
期刊：Phytotherapy Research
DOI：10.1002/ptr.7154
影響因子 ：5.878
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Cell adhesion molecule 4 suppresses cell growth and metastasis by inhibiting the Akt signaling pathway in non-small cell lung cancer(2020.06)
作者：Fang Luo, Yi Zhao, Jiwei Liu
期刊：The International Journal of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2020.105750
影響因子 ：5.085
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A novel colorimetric and ratiometric fluorescent probe for selective detection of bisulfite in real samples and living cells()
作者：Jin Li, Ying Gao, Haoran Guo, Xiaokang Li
期刊：Dyes and Pigments
DOI：10.1016/j.dyepig.2018.12.009
影響因子 ：4.889
引用產品：CL-0016
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Targeted delivery of quercetin by nanoparticles based on chitosan sensitizing paclitaxel-resistant lung cancer cells to paclitaxel()
作者：Yonghong Wang, Hongli Yu, Saisai Wang
期刊：Materials Science and Engineering: C
DOI：10.1016/j.msec.2020.111442
影響因子 ：4.749
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Optimized Fluorescent Probe for Specific Imaging of Glutathione S‐Transferases in Living Cells and Mice(2020.03.30)
作者：Tian Feng, Shouchang Gai, Haiqing Wei
期刊：Chem Asian J
影響因子 ：4.568
引用產品：CL-0016
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Elevated Notch1 enhances interleukin-22 production by CD4+ T cells via aryl hydrocarbon receptor in patients with lung adenocarcinoma(2018.11.25)
作者：Bo Pang
期刊：Bioscience Reports
影響因子 ：3.840
引用產品：CL-0016
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Artesunate inhibits lung cancer cells via regulation of mitochondrial membrane potential and induction of apoptosis(2020.07.15)
作者：Yan Zhao, Jianghui Liu
期刊：Molecular Medicine Reports
影響因子 ：2.952
引用產品：CL-0016
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Oxymatrine?mediated maturation of dendritic cells leads to activation of FOXP3+/CD4+ Treg cells and reversal of cisplatin?resistance in lung cancer cells(2019.03.20)
作者：Hui Liu Manman Zou Pei Li
期刊：Molecular Medicine Reports
影響因子 ：2.952
引用產品：CL-0016
