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產品概述
| 名稱 | 293T [HEK-293T](人胚腎細胞) (STR鑒定正確) |
| 別稱 | Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo |
| 種屬 | 人類 |
| 年齡（性別） | 胎兒 |
| 組織來源 | 胚胎腎 |
| 生長特性 | 貼壁細胞 |
| 細胞形態 | 上皮細胞樣 |
| 背景描述 | 293T細胞是293 [HEK-293]細胞株插入了SV40 T-antigen的溫度敏感基因形成的高轉衍生株。 |
| 生物安全等級 | 1 |
| 生長培養基 | DMEM＋10% FBS＋1% P/S |
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2~3次/周 |
| 倍增時間 | ~24-30小時 |
| 凍存條件 | 凍存液：55% 基礎培養基+40%FBS+5%DMSO 溫度：液氮 |
| 培養條件 | 氣相：空氣，95%；CO2，5% 溫度：37℃ |
| 注意事項 | 該細胞貼壁松散，操作時請盡量輕柔；換液時需預熱培養基；收貨如有大塊脫落的細胞團，為正?，F象，請按照收貨注意事項處理。 |
| 保藏機構 | ATCC; CRL-3216 DSMZ; ACC-635 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 11,12 D2S1338 19 D3S1358 15,16,17 D5S818 8,9 D7S820 11 D8S1179 11,12,14 D13S317 12,14 D16S539 9,13 D18S51 17,18 D19S433 17,18 D21S11 28,30.2 FGA 23 PentaD 9,10 PentaE 7,15 TH01 7,9.3 TPOX 11 vWA 16,19 D6S1043 11 D12S391 19,21 D2S441 11,15 -
STR鑒定圖
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參考文獻
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A Novel Micropeptide Encoded by Y-Linked LINC00278 Links Cigarette Smoking and AR Signaling in Male Esophageal Squamous Cell Carcinoma(2020/07/01)
作者：Siqi Wu, Liyuan Zhang, Jieqiong Deng
期刊：Cancer Research
DOI：10.1158/0008-5472.CAN-19-3440
影響因子 ：12.701
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Inhibition of TPL2 by interferon-α suppresses bladder cancer through activation of PDE4D(2018/11/27)
作者：Qiang Zhe, Zhou Zong-yuan, Peng Ting,
期刊：Journal Of Experimental & Clinical Cancer Research
影響因子 ：11.161
-
Ubiquitination-mediated degradation of SIRT1 by SMURF2 suppresses CRC cell proliferation and tumorigenesis(2020/5/2)
作者：Le Yu, Ling Dong, Hui Li
期刊：Oncogene
影響因子 ：9.867
-
Persistent Luminescence Nanoplatform with Fenton-like Catalytic Activity for Tumor Multimodal Imaging and Photoenhanced Combination Therapy(2020/05/15)
作者：Shuqi Wu, Zihan Qiao, Yang Li
期刊：ACS Applied Materials & Interfaces
影響因子 ：9.229
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MiR-337–3p suppresses proliferation of epithelial ovarian cancer by targeting PIK3CA and PIK3CB(2019/10/17)
作者：Zhen Zhang, Luoyan Zhang, Bin Wang
期刊：Cancer Letters
DOI：10.1016/j.canlet.2019.10.021
影響因子 ：8.679
引用產品：CL-0005
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CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-α in TMJ osteoarthritis(24 April 2020)
作者：Huimin Zhu, Yihui Hu, Chuandong Wang
期刊：Cell Death & Disease
影響因子 ：8.469?
引用產品：CL-0005
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MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells(2021/7/15)
作者：Shu-Hua Fu, Mei-Chen Lai, Yun-Yao Zheng
期刊：Cell Death & Disease
DOI：10.1038/s41419-021-03956-6
影響因子 ：8.469
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MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis(2021/4/6)
作者：Fengxia Chen, Qingqing Wang, Xiaoyan Yu
期刊：Cell Death & Disease
DOI：10.1038/s41419-021-03661-4
影響因子 ：8.468999999999999
引用產品：CL-0005 CL-0041 CL-0114 CL-0150A CL-0150B CL-0380 CL-0525 PM150312B
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Sirtuin2 enhances the tumoricidal function of liver natural killer cells in a mouse hepatocellular carcinoma model(2019.04.06)
作者：Ming Chen, Min Xu, Chengliang Zhu, Hongling Wang
期刊：CANCER IMMUNOLOGY IMMUNOTHERAPY
DOI：10.1007/s00262-019-02337-5
影響因子 ：6.968
引用產品：CL-0005
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ZBTB20-mediated titanium particle-induced peri-implant osteolysis by promoting macrophage inflammatory responses(4th May 2020)
作者：Junxiong Qiu, Peng Peng, Min Xin, Zhenkang Wen
期刊：Biomater. Sci.
影響因子 ：6.843
引用產品：CL-0005
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microRNA-27b shuttled by mesenchymal stem cell-derived exosomes prevents sepsis by targeting JMJD3 and downregulating NF-κB signaling pathway(2021/1/7)
作者：Jia Sun, Xuan Sun, Junhui Chen
期刊：Stem Cell Research & Therapy
DOI：10.1186/s13287-020-02068-w
影響因子 ：6.832
引用產品：CL-0005
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MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7(2020.01)
作者：Lili Lou, Mengyuan Tian, Jingxia Chang
期刊：Biomedicine & Pharmacotherapy
DOI：10.1016/j.biopha.2019.109692
影響因子 ：6.529
引用產品：CL-0005
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Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1(2020/10/22)
作者：Zhiwei Zhang, Zihan Cui, Zhuolin Xie
期刊：Translational Lung Cancer Research
影響因子 ：6.498
引用產品：CL-0005
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miR-19a-3p downregulates tissue factor and functions as a potential therapeutic target for sepsis-induced disseminated intravascular coagulation(2021/7/9)
作者：Rong Zhang, Sifen Lu, Xudan Yang
期刊：Biochemical Pharmacology
影響因子 ：5.858
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MiR-185-5p ameliorates endoplasmic reticulum stress and renal fibrosis by downregulation of ATF6(2020.06.08)
作者：Quan Yuan, Tianhua Xu, Ying Chen
期刊：Laboratory Investigation
影響因子 ：5.662
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MiR-126-3p-Enriched Extracellular Vesicles from Hypoxia-Preconditioned VSC 4.1 Neurons Attenuate Ischaemia-Reperfusion-Induced Pain Hypersensitivity by Regulating the PIK3R2-Mediated Pathway(2020/10/08)
作者：Wang He, Chen Feng Shou, Zhang Zai Li
期刊：Molecular Neurobiology
DOI：10.1007/s12035-020-02159-y
影響因子 ：5.59
引用產品：CL-0005
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A novel surface modification method upon halloysite nanotubes: A desirable cross-linking agent to construct hydrogels(2019/8/13)
作者：Yuangong Zhang, Libin Bai, Cong Cheng
期刊：Applied Clay Science
DOI：10.1016/j.clay.2019.105259
影響因子 ：5.467
引用產品：CL-0005
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MicroRNA-205-5p targets E2F1 to promote autophagy and inhibit pulmonary fibrosis in silicosis through impairing SKP2-mediated Beclin1 ubiquitination(2021/08/27)
作者：Qingzeng Qian, Qinghua Ma, Bin Wang
期刊：Journal Of Cellular And Molecular Medicine
影響因子 ：5.31
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Protective mechanism of SIRT1 on Hcy‐induced atrial fibrosis mediated by TRPC3(04 November 2019)
作者：Lu Han, Yanhua Tang, Shaochuan Li
期刊：Journal of Cellular and Molecular Medicine
影響因子 ：5.310
引用產品：CL-0005
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miR‐129‐2‐3p directly targets SYK gene and associates with the risk of ischaemic stroke in a Chinese population(2018/11/29)
作者：Suli Huang, Ziquan Lv, Ying Wen
期刊：Journal Of Cellular And Molecular Medicine
影響因子 ：5.31
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Resveratrol ameliorates the glucose uptake and lipid metabolism in gestational diabetes mellitus mice and insulin-resistant adipocytes via miR-23a-3p/NOV axis(2021/07/10)
作者：Tao Zheng, Hainan Chen
期刊：Molecular Immunology
DOI：10.1016/j.molimm.2021.06.011
影響因子 ：4.407
引用產品：CL-0005
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RP11?284F21.9 promotes lung carcinoma proliferation and invasion via the regulation of miR?627?3p/CCAR1(August 12, 2020)
作者：Dan Li, Li Wang, Jin Feng, Yan?Wei Shen
期刊：Oncology Reports
影響因子 ：3.906?
引用產品：CL-0005
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MiR-384 induces apoptosis and autophagy of non-small cell lung cancer cells through the negative regulation of Collagen α-1(X) chain gene(FEBRUARY 01 2019)
作者：Ye Xu; Ning Wang; Wen Zhao
期刊：Biosci Rep
影響因子 ：3.840
引用產品：CL-0005
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MiR-130a regulating the biological function of colon cancer by targeting inhibition of PTEN()
作者：H.-L. LIU, H.-G. BAO, C.-L. ZHENG, C. TENG, M.-H. BAI
期刊：European Review for Medical and Pharmacological Sciences
影響因子 ：3.507
引用產品：CL-0005
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MiR-124-3p inhibits the migration and invasion of Gastric cancer by targeting ITGB3(2020.01)
作者：Qian Wu, Huiyu Zhong, Lin Jiao, Yang Wen
期刊：Pathology - Research and Practice
影響因子 ：3.250
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miR-34a-5p Inhibits Cell Proliferation, Migration and Invasion Through Targeting JAG1/Notch1 Pathway in HPV-Infected Human Epidermal Keratinocytes(2019.11.28)
作者：Ying Gao, Ming Yang, Liuliu Wei
期刊：Pathology & Oncology Research
DOI：10.1007/s12253-019-00782-2
影響因子 ：3.201
引用產品：CL-0005
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VDAC1 is regulated by BRD4 and contributes to JQ1 resistance in breast cancer(2019.0627)
作者：Guochao Yang, Dianwei Zhou, Jun Li Wei Wang
期刊：Oncology Letters
影響因子 ：2.967
引用產品：CL-0005
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Silencing of MALAT1 inhibits migration and invasion by sponging miR?1?3p in prostate cancer cells(2019.08.22)
作者：Xiaofan Dai, Zuowen Liang, Lingyun Liu, Kaimin Guo, Shengqi Xu
期刊：Molecular Medicine Reports
影響因子 ：2.952
引用產品：CL-0005
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Krüppel?like factor 4 ameliorates diabetic kidney disease by activating autophagy via the mTOR pathway(2019.08.09)
作者：Jianguang Gong, Huifang Zhan, Yiwen Li, Wei Zhang
期刊：Molecular Medicine Reports
影響因子 ：2.952
引用產品：CL-0005
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Effects of PYRIN-containing Apaf1-like protein 1 on isoflurane-induced postoperative cognitive dysfunction in aged rats(2020.06.16)
作者：Xiaona Zhang, Xiushuang Fan
期刊：Molecular Medicine Reports
影響因子 ：2.952
引用產品：CL-0005
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Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor?κB activity(2019.02.19)
作者：Lingling Sun, Chengyu Sun
期刊：Molecular Medicine Reports
影響因子 ：2.952
引用產品：CL-0005
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